CONSOLIDATED
PRACTICUM
TECHNICAL
APPLICATIONS LABORATORY TEST
METHOD
CARBOHYDRATE IODIN
NAME
: ERVAN, TOGATOROP
NIM
: G31113302
GROUPS:
FIVE
ASSISTANT
: DEWI SARTIKA MONOARFA
CHEMICAL
LABORATORY ANALYSIS AND QUALITY CONTROL
PROGRAM
FOOD SCIENCE AND TECHNOLOGY
DEPARTMENT
FOOD
HASANUDDIN
UNIVERSITY MAKASSAR AGRICULTURAL TECHNOLOGY
2014
I. INTRODUCTION
KEP.I.1 /background
carbohydrate solution is needed in our bodies.
Carbohydrate is the compound macromolecule that was found in food that
consisted of the elements carbon, hydrogen and oxygen. Carbohydrate consists of
four parts are monosaccharide, disakarida, oligosakarida and polysaccharide.
Monosaccharide consists of one cluster sugar, disakarida consists of two
clusters sugar, oligosakarida consists of three to ten cluster sugar,
polysaccharide consists of ten or more cluster sugar. Each cluster sugar
dihubungan by ikatan gliosidik.
Their carbohydrates function as that are the
main energy in the body. For activity we need energy, energy, from the
materials food that contains carbohydrates. Main sources are carbohydrates that
very often, we know such as rice, corn, potatoes, cassava, sago. Carbohydrate
Analysis can be done in qualitative and quantitative. Quantitative Analysis is
used to analyze number of carbohydrates in food. Quantitative analysis example
is the method lowry. Qualitative Analysis in use to analyze is tidanya carbohydrates
in food. Conto qualitative analysis method is iodin.
Carbohydrate qualitative Analysis is done in
order to know whether there exists carbohydrates in food. The method iodin can
be used to analyze carbohydrates in qualitative research. Types of carbohydrate
in test with the method karbohidat is carbohydrate types of polysaccharide.
Polysaccharide added iodin will set up a different colors according to the type
of lots and lots.
I.2 aims and
objectives he did practicum test functionality
carbohydrate iodin method is as follows
1. To find out the principles testing method
with carbohydrate iodin
2. To find out what kind of carbohydrates in a
food in qualitative research.
He uses practicum testing karohidrat with ioin
method is so that everyone practicum understand how to test carbohydrates in
qualitative research and has gone types of carbohydrates that are in ujinya.
II. LITERATURE review
II.1 Potato (Solanum Tuberosum)
was a plant which contains carbohydrates. Plants
debt, not sycamore, like a mild such as in the highlands hill (). Was a
plant (the flowers in bloom perfect) and has been prepared compound with size
around 3 cm. Potato colors ranging from chocolate purplish patches to yellowish
white (Ilias, 2012).
Types of potato based Distan (2012) is as
follows.
Wuhan : Plantae
Subkingdom : Tracheobionta
Super Division : Spermatophyta
Division : Magnoliophyta
Class : Magnoliopsida
Sub-Class : Asteridae
orders : Solanales
family : Solanaceae
Genus : Solanum
species : Solanum tuberosum L
Potato is famous because the content of
carbohydrate him (around 26 gram in potato medium). Dominant form of
carbohydrate is pati. Some small but significant pati is resistant to digestive
system by the enzymes in the stomach and small intestine, so that it can reach bowel
essentially intact (anonymous, 2010)
II.2 Carbohydrate
Carbohydrate are polysaccharide, is a main
source of energy to food. Rice, cassava, corn are just a few examples food
contains carbohydrates. Building Blocks page carbon, hydrogen carbohydrate are,
and oxygen (C, H, O) with the formula general Cn(H2O)n. Because this is the
name carbohydrate given. Carbohydrate comes from the word 'carbon' and
'hydrous'. Carbon Atoms that binds water (Haris, 2013).
The grouping carbohydrate according to Selastini
(2011) that is: 1
.
A Simple carbohydrates. Monosaccharide
consists of atoms C period with a molecule
water, namely {C6(H2O)6} and {C5(H2O)5}. Most monosaccharide known as
heksosa, because consisting of 6- chain or a ring carbon. Atoms - atoms
hydrogen and oxygen is bound in chains or ring was separately or as cluster
hydroxyl ( o ). There are three kinds of heksosa is important in
nutrition, namely glucose, fructose and galaktosa. The three kinds monosaccharide
contain numbers and types of same atoms, namely 6 carbon atoms, 12 atoms
hydrogen, and 6 oxygen atom. The difference is located in the way atoms - atoms
hydroThe difference is located in the way atoms - atoms hydrogen and oxygen
around atoms - carbon atoms. The difference in the order an atom this is what
causes the difference between the high. sweetness, power is dissolved, and the
attributes another third monosaccharide. Monosaccharide that was found in
nature in generally found in the form dekstro handed isomers.
2.
There are four different types of Disakarida
disakarida, namely sucrose or sakrosa, maltose, lactose and trehalosa.
Disakarida consists of two units monosaccharide is tied with one another
through condensation reaction. Disakarida can be driven back to two molecules
monosaccharide through reaction hidrolisis. Glucose is found in the four types
of disakarida, monosaccharide other is fructose and galaktosa.
3. Oligosakarida
Oligosakarida consists of polymer two to ten
monosaccharide. Rafinosa, stakiosa and verbaskosa is oligosakarida that
consists of unit - unit glucose, fructose and galaktosa. Fruktan is a group
oligo and polosakarida composed of several units fructose is tied with one
molecule glucose. Fruktan is found in cereal, onion, garlic, and asparagus.
4. Polysaccharide
Polysaccharide which comprises more than two
ikatan monosaccharide. Carbohydrate complex can contain up to three thousand
unit sugar that simple long chain arranged in the form straight or branch.
Types of polysaccharide which are important in nutrition, pati, dektrin,
glycogen and polysaccharide nonpati.Fiber named also polysaccharide nonpati
.two fiber, namely that cannot be dissolved and
that can be dissolved in water. Fiber that is
not fully dissolved in water is a blatantly from cellulose, hemicellulose and
lignin. Fiber that is dissolved in water is pectin which was an antioxidant
essence, gum, mukilase, glukan and algal.
II.3 Carbohydrate test method Iodin
Condensation iodin with carbohydrates in trial
iodin, monosaccharide can produce colors that is unique. This was because the
solution pati, there are units glucose that formed a chain helix because of
ties with configuration on each unit glukosanya. This form causes pati can form
a complex molecule iodine that is able to fit in, causing sticky spirals dark
blue in the complex (Fessenden, 1986).
Solution amilum after ditetesi iodine (before
heat) solution with white nodes. However, after heat colors solution is still
white nodes but there is a sludge purple tube go any reaction. This shows that
this happened hidrolisis pati at global warming. The sediment that appeared in
the bottom of the jar is partly due to the fact that the process hidrolisis
pati that was not perfect. This is the rest of The details of the
amilum (Divans, 2012).
Bond between iod and amilum as ikatan illusion
because they can be broken when heat and formed again at the moment is cooled.
When heat chain amilum lengthen so iod, just as easily despite when cooled, the
chain amilum will contract so iod again tied with amilum. This is because
menghidrolisis ability so that amilum turned into a glucose. Testing amilum
will be done in the atmosphere acid, base and neutral. Additional solution iod
0.01 M in water in the atmosphere base does not happen color change because iod
not bind to amilum (Sherly, 2012).
Pati and iodine bond complex blue color. Pati in
the atmosphere acid when when heat can terhidrolisis to the compound that more
simple, the results were tested with iodine that will give the blue and not in
color. If amylose direaksikan with iodine and it will be a blue, while if
amilofektin direaksikan with iodine will give color purple blackness (Mustaqim,
2012).
Amilum reacted with molecules iod because the
structure amilum solution in
helical shaped like a spool so that it can be
completed by this molecule iod
in it. However, after global warming, the color
solution to nodes.
This was because there is a decisive break ties
with glucose Iod earlier or from fragmenting ion (authorization iod from amilum)
because the change in temperature. After cooled, solution back blue color. This
shows that this bond between
iod and amilum as ikatan illusion because they
can be broken when heat and formed again at the moment is cooled (Raandesky,
2011).
Trial hidrolisis pati by acid, in the minute 0
after samples ditetesi solution iodin samples blue glaze. In the minute 4
teaching Global warming and ditetesi iodin solution, the color samples is still
livid. Global Warming after minutes to 8 and ditetesi iodin solution, the
sample blue keabuan or memudarnya blue color glaze. In the minute 12 global
warming color samples fade away to blue keabuan after ditetesi iodin. After the
warming up session 16 minutes and ditetesi iodie, color samples to clear
(Grace, 2012).
Trial iodin used for medeteksi the pati (a
polysaccharide), when done experiment with three conditions that condition,
neutral, acid and bases, which are at each tube added 2 drops in water tubes I
(neutral), 2 drops HCl in tubes II (acid) and 2 drops Na OH in tubes III
(base). And Then the three tubes, after heat in a tube I with a neutral,
( 2 drops water) did not happen color change, with base ( 2 drops Na OH) has
not changed colors (colors is still muddy) or in other words, not formed ikatan
coordination between ion iodida at helix.
This is because the base I2 will experience a
reaction as follows:
3 I2 6 Na OH → 5 NaI naio3 3 H2O
So that the solution is not I2 causing the
occurrence of ikatan coordination and therefore colors is still muddy, while
with the current acid
(Na OH II.4 Sodium Hydroxide
(Na OH) Sodium hydroxide, also known as soda
kaustik or sodium hydroxide, is a kind base volume kaustik. Sodium hydroxide
formed darioksida base Sodium oxide dissolved in water. Sodium hydroxide
solution alkaline formed a strong when dissolved into the water. Sodium
hydroxide solid and pure form of white available in the form pelet, crumb, a
small object or a saturated 50 percent. Sodium hydroxide is moist and liquid
spontaneously absorb carbon dioxide from free air. Na OH is dissolved in water,
to deliver warm when being dissolved. Na OH also dissolved in ethanol and
methanol, even though solubility Na OH in the balance is smaller than
solubility KOH (anonymous, 2012).
Additional Na OH function is to give the
atmosphere base on the try iodin. In testing solution amilum and iod' Na OH
preclude a reaction between amilum with iod. This is because iod reacted with
base so that it does not have a reaction with amilum. This situation occurred
because Na OH is the solution first to react with iod form compound NaI and
naoi" so that the trial with additional Na OH not there was a change in
solution amilum (anonymous, 2011).
II.5 Aquadest
Aquades or common in call distilled water is the
water purification result (been evaporated and disejukan again).distilled water
also has a chemical formula in water that is generally H20 which means in 1
molecules there are 2 hydrogen atoms covalent bond and oxygen atom single. H20
molecules in form of asymmetrical. Because water molecules asymmetrical and
oxygen atom has electronegativity higher than hydrogen atoms, he brought
negative (-) charge a little, while hydrogen atoms little positive. As a
result, the water is a molecule polar moment with dipol electricity or is not
the same as 0. Water can also be set up in a big number hydrogen bond
intermolecular force for a molecule size (anonymous, 2011c).
Aquades is water destilasi or refiners with
pure water or H20, because H20 almost does not
contain minerals. While mineral water is solvent universal. Additional akuades
the establishment carbohydrate iodin method is as larutannetral (anonymous,
2011).
II.6 hydrochloric acid (HCl) Adding
HCl in testing carbohydrate had the same
function like other reagent, HSO4. Both work for menghidrolisis polysaccharide
to monosaccharide immense. Amilum that has been added with hydrochloric acid
when tested with iodine solution, in a negative impact, it can be concluded
that amilum has terhidrolisis with perfect (Sativa, 2008).
III. The method PRACTICUM
III.1 time and place
during the announcement carbohydrate iodin
method was done on wednesday, november 12, 2013, at 08.00 - 12.00 WITA Chemical
laboratory analysis, and Quality Control study program, Food Science and
Technology Food, majoring in Agricultural Technology, the Faculty of Agriculture,
Hasanuddin University, Makassar.
III 2 equipment and materials
tools used in this practicum:
- a knife - hot plate
- batang stirring spoon or rod - a glass
chemical
pipette measure - container
- stopwatch - weight analytic
- rak tube reaction - clipper tube reaction
- scar - shaker bottle
materials used in this practicum were as
follows:
- aquadest - potato
- HCl 3% - corn
- Na OH 6 M - bananas
- sweet potato-flour
III.3 procedures Practicum
1. Material skinned and being washed clean and
crushed by using blender/grated.
2. Weighed as much as 5 grams with scales
analytic.
3. Put into the glass a measure and are added
aquades 50 ml.
4. Material into the 3 tube reaction. Each 3 ml
then given equal treatment and to be observed color change that occurs. But his
offer:
- aquades 2 drops
- solution HCl 3% 2 drops
- solution Na OH 6 M 2 drops
5. Each sample added solution iodin 3 drops and
color change that occurs carefully.
6. When Heat with penangas/hot plate temperature
at 60 for 5 minutes.
7. Redundancy samples during 10 minutes later
color change look at what happened.
IV. RESULTS and discussion
results obtained IV.1 result from practicum
trial carbohydrate iodine methods can be seen in the table below:
table 12. Test result Carbohydrate additional
Aquadest
No material treatment
Aquadest Iodin Heat Cooled
1 Potato White White White White -
2 Papaya Yellow Yellow has faded Yellow has
faded Yellow has faded
3 banana dirty White White muddy dirty White
White -
4 sweet potato White-gray Nodes Nodes
5 Corn Yellow muddy dark yellow or Yellow Yellow
- A Source
: The data Primary Practicum Technical Applications
Laboratory 2014.
Table 13. Test result Carbohydrate additional
HCl 3%
No material treatment
HCl Iodin Heat Cooled
1 Potato dirty White Blue Blue - Blue
2 Papaya Yellow yellow light yellow - Yellow
faded away - -
3 banana dirty White Blue glaze White White -
4 sweet potato White Blue Nodes Nodes
5 Corn Yellow muddy dark yellow or Yellow Yellow
- A Source
: The data Primary Practicum Technical
Applications Laboratory 2014.
Table 14. Test result Carbohydrate additional Na
OH
No material treatment
Na OH Iodin Heat Cooled
1 Potato Yellow Yellow and White White -
2 Papaya bright yellow color bright yellow color
bright yellow color bright yellow color -
3 banana Yellow muddy Yellow muddy bright yellow
color - Yellow muddy - -
4 sweet potato Yellow Yellow Nodes yellowish -
Nodes yellowish
5 Corn Yellow muddy Yellow muddy yellow light
yellow light
Source: Data Primary Practicum Technical
Applications Laboratory 2014.
V. iv.2 ). discussion
materials to use one group in practicum trial
carbohydrates with iodin method is potatoes 5 gram. Potato contains quite a
high carbohydrate. In 100 grams potato there are approximately 19 grams
carbohydrate. Types of carbohydrates that are generally found in potato is
carbohydrate polysaccharide namely pati. Pati consists of amylose and
amylopectin. This is in accordance with the anonymous (2011) stated that
potatoes well-known because it contains lots and lots. Dominant form of
carbohydrate is pati. Some small but significant, pati is resistant to
digestive system by the enzymes in the stomach and small intestine, so that it
can reach bowel essentially intact. The content of carbohydrate potatoes per
100 g is 19 g.
Tube first reaction included 5 mL material that
is white and included 3 drops aquadest and dihomogenkan. Aquadest that is added
to the material was neutral and transparent so that it does not affect
material. Aquadest function as a solvent segingga could not be menghidrolisis
glucose. This is in accordance with Yudhisthira (2011) is a aquadest or
aquadestilata or water denim is the water that has been purified. In addition
aquades solution karohidrattidak resulting in a change.
Additional iodin done after adding aquades in
previous treatment. Iodin added in a sample as many as three drops and
dihomogenkan. Results obtained from treatment is solution is still a white
House muddy. It should be adding iodin resulting in changes color of the
solution. This was because of the lack of precision in seeing color change that
occurs. The formation blue color in a carbohydrate because amilum on potatoes
to react with iodin. This is in accordance with the cloud (2011) that iodin
Added a change color to sample carbohydrates. Amilum on potato that
spiral-shaped makes it easier for iodin check-in easily.
Treatment in a first reaction was heat for 5
minutes above penangas with the temperature 60o C and cooled for 10 minutes.
Results obtained when heat is a white color. This is because ikatan amilum
formed long chain at the time when heat. When a potato cooled, solution back to
color again. This is because ikatan amilum back has been established. This is
in accordance with Diwan (2012) stated that, bond between iod and amilum can
lose when heat and formed again at the moment is cooled.
A Tube reaction both included 5 mL samples and
then added HCl 3% in tubes and dihomogenkan. It was observed reactions that
occur. What happens is solution to the white. This was diesbabkan because HCl
is amino acid that additional form HCl slash pH samples. Revelation pH samples
is marked with color change. This is in accordance with the Adiatma (2011)
amilum spiral-shaped so that it is easy to bind to iod.
After added HCl tube reaction both added iodin
as much as 5 drops and dihomogenkan. After that, watched by reactions that
occur. Iodin to indicators that show color change in samples. Colors are formed
is blue color. This was because of the reactions between amilum with iod. Pati,
the atmosphere acid so amilum will terhidrolisisdan is easy they bind to iod to
form the color blue. This is in accordance with the cloud (2011) that iodin
added functions as a indicators polysaccharide compounds.
Treatment in tubes second reaction is, the
sample heat for 5 minutes above penangas with the temperature 60 and cooled for
10 minutes. When heat solution blue color in solution decreases. This is
because when samples when heat and amilum lengthen so iod is easy. When cooling
solution back to color again. This is because a chain amilum back contract.
This is in accordance with the sherly (2012) stated that the bonds between iod
and amilum a bond illusion. So that it is easy to change depending treatment
that will be given.
The third reaction tube samples included as many
as 5 mL then put Na OH 6 M as many as 3 drops and dihomogenkan. After that, it
was observed reactions that occur. A change to yellow color. This is because
the base dissolved in water so that it will deliver heat. When dissolved into
colors yellow. This is in accordance with an Anonymous (2012) stated that
additional Na OH function is to give the atmosphere base on trial iodin.
Additional Na OH causes color change to yellow color.
Iodin added as much as 5 drops into a tube
reaction that has been added Na OH 6. Additional iod did not cause any changes.
This is because iod ahead of time to react with Na OH so that there was no
reaction between iod
with amilum. A reaction between Na OH with Iod
will set up a compound NaI and naoi. This is in accordance with the cloud
(2011) stated that the testing solution amilum and iod, Na OH preclude a
reaction between amilum with iod. Iod first to react with Na OH that amilum
with Na OH.
Tube Advanced reaction third heat for 5 minutes
at high temperature 60 and cooled during 60 and then cooled for 10 minutes.
Results obtained when heat, did not experience any changes so after cooled.
This was because there was no reaction to the beginning of amilum with iod. So
that when there is no ties that warming up and has been established. This is in
accordance with the Raandesky (2011) stated that the treatment such as global
warming and cooling solution in base that has been added iod did not experience
any changes.
V. The closing
XIX.V.1 conclusion
that could be obtained from practicum is as
follows:
1. Content of carbohydrate in a food can be
known by doing a qualitative testing method trial iodin.
2. The principle of the announcement
carbohydrates with test method iodin to identify polysaccharide. Reagent is
iodine solution. Amilum with iodine can form a complex blue, Amylopectin with
iodin will give a color scarlet while with glycogen and dekstrin will set up a
bright red brown
V.2 Question and
suggestions that can be advanced practicum
praktikan gave to the advanced practicum should be able to run more
efficiently, and ensured that there is no uproar when process practicum took
place.
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